High-pressure processing-induced transcriptome response during recovery of Listeria monocytogenes

Abstract Background High-pressure processing (HPP) is a commonly used technique in the food industry to inactivate pathogens, including L. monocytogenes . It has been shown that able recover from HPP injuries and can start grow again during long-term cold storage. To date, gene expression profiling of damage recovery at cooling temperature not studied. In order identify key genes play role caused by treatment, we performed RNA-sequencing (RNA-seq) for two strains (barotolerant RO15 barosensitive ScottA) nine selected time points (up 48 h) after treatment with pressure levels (200 400 MPa). Results The results showed general stress response was activated SigB treatment. addition, phosphotransferase system (PTS; mostly fructose-, mannose-, galactitol-, cellobiose-, ascorbate-specific PTS systems), protein folding, cobalamin biosynthesis were most upregulated recovery. We observed cell-division-related ( divIC , dicIVA ftsE ftsX ) downregulated. By contrast, peptidoglycan-synthesis murG murC pbp2A upregulated. This indicates cell-wall repair occurs as part also prophage genes, anti-CRISPR induced HPP. Interestingly, large amount RNA-seq data 85%) mapped Rli47, which non-coding RNA Thus, predicted Rli47 plays Moreover, gene-deletion experiments amongst peptidoglycan mutants are more sensitive Conclusions identified several mechanisms may Our study contributes new information on pathogen inactivation